Bioimaging & Flow Facility
The Bioimaging and Flow Cytometry Facility incorporates all of the Institute’s high-end microscopy, cell sorting and flow cytometry equipment. Microscopes include upright and inverted multiphoton, confocal and widefield with SHG, phase contrast, DIC, timelapse, multiposition and Class II live pathogen imaging capabilities available. Explore some of the systems and resources they offer users below, and be sure to check out their website for full details and the most up-to-date information.
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The Roslin Institute,
Easter Bush Veterinary Campus,
Midlothian,
EH25 9RG
- Anna Raper
- Anna.Raper@roslin.ed.ac.uk
- +44 (0)131 6519373
Microscopes
| Microscope | Upright or inverted | Timelapse | Multiposition | HO Licensed | Class II Pathogen | Other features | ||
|---|---|---|---|---|---|---|---|---|
| Zeiss LSM 880 Airy & Fastscan | Inverted | ✔ | ✔ | |||||
| Zeiss LSM 710 Confocal | Inverted | ✔ | ✔ | |||||
| Zeiss LSM7 Multiphoton | Upright | ✔ | ✔ | SHG, Coherent 690-1350nm laser with OPO | ||||
| Zeiss Live Cell Observer | Inverted | ✔ | ✔ | ✔ | Deconvolution, Epifluoresence, Widefield, Phase-contrast, DIC | |||
| Zeiss Lightsheet 7 | N/A | ✔ | ✔ | has live and cleared imaging capabilities. 4 lasers and dual cameras | ||||
| Leica DMLB, DMLB2, DMRB Epifluorescent Microscopes | Inverted | 3 Available | ||||||
| Nikon Ni1, E600, E400A Brightfield Microscopes | Inverted | 4 Available | ||||||
| Zeiss Axiovert 25 & 40 | Inverted | 2 Available | ||||||
| Zeiss StereoLumar V12 Stereomicroscope | ||||||||
| Bigfoot Cell Sorter | The Bigfoot is Scotland’s first spectral cell sorter. It has 57 channels, is fast and flexible as to sorting media. It can sort 1 cell into a 384 well plate in 11 seconds. It is also BCLII compliant. |
Image Analysis
- ICY
- Imaris
- FIJI/ImageJ
Additional Resources
- Assisted imaging sessions
- Remote imaging by facility staff
- Access to tissue-culture facilities
- Access to incubators
- Access to dissecting microscope
- Sample preparation
- Managed online data storage