Institute for Regeneration and Repair (IRR) Imaging Facility

The facility offers a wide range of microscopes and imaging techniques.  We can image samples ranging from a whole bee down to single molecules.  We are equipped for live, multiposition imaging as well as fixed samples.  Techniques on offer include FLIM, holotomography, multiplexing, phase-contrast, superresolution.  We have a microfabrication unit to pattern cell culture substrate, as well as create 3D gels for cell culture and microfluidic device design. The facility offers several dedicated high-end workstations for bio-image processing and analysis. Explore some of the systems and resources they offer users below, and be sure to check out their website for full details and the most up-to-date information.

  • IRR Imaging Facility,
    Institute for Regeneration and Repair (South)
    Edinburgh BioQuarter
    4-5 Little France Drive,
    Edinburgh,
    EH16 4UU
Chrome
MicroscopeUpright or invertedTimelapseMultipositionHO LicensedClass II PathogenOther features  
Bioptonics OPT 3001M Optical Projection tomography✔✔3D imaging of large samples in brightfield and GFP/RFP fluorescence
Nikon AZ100 MacroscopeUpright✔✔✔✔Brightfield and fluorescence in DAPI, GFP, RFP, Cy5; multiple zoom; 1x, 2x and 3x lenses; motorised stage
Leica MF165 FC MacroscopeUpright✔Colour imaging, multiple levels of zoom
Nikon Ti WidefieldInverted✔✔✔Phase contrast, fluorescence, JOBS
Nikon Ti-2 WidefieldInverted✔✔Microfabrication with Alveole’s PRIMO, nanoindentation with OpicsLife11 Chiaro
Zeiss Observer Z1 WidefieldInverted✔✔✔✔Phase contrast, flexible fluorescence through excitation and emission filter wheels, AI sample finder, Zeiss Connect
Zeiss Observer WidefieldInverted✔✔✔Phase contrast, fluorescence, colour and greyscale cameras
Leica DMi8 WidefieldInverted✔✔Phase contrast, fluorescence, colour and greyscale cameras
Leica DM2500 WidefieldUpright✔✔Phase contrast, fluorescence, colour camera
Akoya Phenocycler Multiplex Scanning SystemInverted✔Up to 100 antibodies on the same sample
Leica SP8 4DInverted✔✔✔✔Long working distance lenses, 4 laser lines
Leica SP8 5D ConfocalInverted✔✔✔✔Long working distance lenses, 9 laser lines, resonant scanner for fast acquisition, FRAP, FRET
Leica FALCON ConfocalInverted✔✔✔✔Tuneable white light laser, pulsed laser for FLIM, long working distance lenses, , FRAP, FRET
Zeiss Elyra SuperresolutionInverted✔✔✔✔Structured illumination in Apotome mode (low magnification, 5-40x) and SIM mode (high magnification). TIRF, single molecule modes
Alveole PRIMO MicropatterningInverted✔Substrate micropatterning, 3D gel curing, part of microfabrication suite for substrate engineering and microfluidic design
OpticsLife11 Chiaro NanoindentationInverted✔Measures tissue stiffness and stickiness
Agilent iCelligence S16 ImpedenceInverted✔Measures cell confluence over time
Labtech X-Clarity Sample ClearingInverted✔Tissue clearing station for samples up to 3cm
Nanolive HolotomographyInverted✔✔✔3D holotomography and 2D fluorescence
Nikon Ts2R-FL 13 TC MicroscopeUpright or Inverted✔Phase contrast, fluorescence, greyscale camera
Particle Metrix Zetaview Particle Analyser✔Designed for small particles like Extracellular Vesicles and viral particles. Measures size, concentration, ZetaPotential, fluorescence in DAPI, GFP, RFP, Cy5 ranges
Commercial:
  • Huygens
  • VisioPharm
  • Leica  AiVia
  • Zeiss ARIVA

Freeware:

  • Fiji
  • QuPath
  • CellProfiler
  • Ilastik
  • Cellpose
  • StarDist
Come to our 11-session course on image analysis, run twice a year
  • Assisted imaging sessions
  • Remote imaging by facility staff
  • Sample preparation
  • Assisted image analysis
  • Remote Image Analysis