Winner of the 2020 Summer Image Competition
The Colour Explosion
Anh Le, CRUK Beatson Institute
This image shows the dynamics of the endocytic network, here labelled by the early endosomal marker RAB5A. Each shade of yellow represents a position in time for each vesicle.
Runners-up
Special Mention
Karthik Krishnamurthy
Thomas Jefferson University
‘Flowering of Neuronal Activity’. Cortical neurons cultured from rat embryos(E17) for 7 days in vitro and labeled with the calcium indicator Fluo-4. Neurons were then stimulated with glutamate and Fluo-4 fluorescence intensity was imaged for 30 minutes at 5 second intervals using a 20X air objective of a nikon Confocal microscope (A1R). Image represents volume view of fluo-4 fluorescence intensity indicative of calcium influx over the 30 minute period blended using depth color coded alpha algorithm as a function of time.
Special Mention
Gabriel Cote
Macro stack at 40x common green blue fly (97 pictures), taken with OMAX MD827S30 microscope and Pixel phone camera.
Kevin Mackenzie
University of Aberdeen
Vitamin C crystals. Taken on a Zeiss polarized light microscope.
Farah Abdulkhaleq
University of Aberdeen
These are 3 stimulated lymphocytes. The one with yellow surface is a CD4+ T cell. Green (goat anti-rabbit IgG (H+L) AF488) and red (streptavidin AF555) channels are CTLA-4 and sCTLA-4, respectively, while the blue channel (DAPI) represents the nucleus. Image was taken using confocal laser scanning microscopy (LSM 880).
The funny thing is that the cells together form the shape of a bee!
Dale Watt
CRUK Beatson Institute
A cancerous duct in a mouse model of pancreatic ductal adenocarcioma surrounded by cancer associated fibroblasts. Imaged on the Nikon A1R at 40x. Stained for aSMA-AlexaFluor 647 and pSMAD3 -AlexaFluor488 and DAPI.
Kevin Mackenzie
University of Aberdeen
Zebra fish embryo taken using a darkfield microscope.
Azita Kouchmeshky
University of Aberdeen
NSC-34, a mouse motor neuron-like hybrid cell line, transiently transfected with the (GFP)-SOD1G93A going through autophagy process near a non transfected cell in autophagy phase labelled by LC3B marker. Image acquired using a Nikon field fluorescence microscope)
Simon Brown
University of Edinburgh
PFA-fixed cross section of adult mouse testes stained with lymphoid specific helicase (LSH), promyelocytic leukemia zinc finger (PLZF), Synaptonemal Complex Protein 3 (SYCP3) and DAPI conjugated to Alexa Fluor secondary antibodies (Fluorophores 488 LSH, 594 PLZF, 647 SYCP3).
Leandro Lemgruber
University of Glasgow
Scanning electron micrograph of gametes of the Malaria parasite. Microgametes (in yellow) are trying to fertilise a macrogamete (in pink).
Madara Brice
University of Edinburgh
Cross-section of the liver of a c-Kit Cre reporter mouse, although the reporter is actually labelling all endothelial cells (as apposed to sub section with c-kit positivity). Yellow stain – cytoplasm of all endothelial cells, blue – DAPI. Picture taken on an AxioScan Slide Scanner.
Iván Coto Hernández
Harvard Medical School
Widefield imaging of myelinated and unmyelinated fibers of peripheral nerve in Sox10-Venus mice labelled with FluoroMyelin Red. The image quality was improved using deconvolution Huygens deconvolution software.