GIF has imaging technologies that cover whole body imaging, conventional wide-field, confocal and multi-photon microscopy, to high-resolution approaches breaking the resolution limit of light. Techniques available are Spinning Disk Confocal, Multiphoton, High Content InCell, Super-Resolution (3D-SIM and STORM), wide-field deconvolution (DV-Core and Leica DMi8), In Vivo systems (IVIS), TEM and the imaging flow cytometer ImageStream.
Diffraction limited: Spinning Disk Confocal
Beatson Advanced Imaging Resource
BAIR have a wide range of specialised microscopes that provide users with the flexibility to approach their research using a diverse range of applications. Microscopes include a variety of confocal and widefield systems, super-resolution systems, high-content screening, in vivo imaging systems, and equipment for advanced techniques such as TIRF, FLIM/FRET and Multiphoton imaging.
GCU Bioimaging
GCU Bioimaging offers confocal and live cell imaging platforms, with LSM800 airyscan and Crest X-light spinning disk-TIRF confocal systems. Dedicated calcium imaging and microinjection rigs are available, as are phase contrast and histological analysis using an Axiovert and EVOS system.
Aberdeen Microscopy and Histology Core Facility
The Microscopy and Histology facility has many years’ experience in Histology (wax, cryostat and resin) and Light, Fluorescence, Confocal along with MicroCT and also Electron Microscopy. Light microscopes include upright and inverted confocal, spinning disk confocal, widefield, phase holographic and slide scanner system with timelapse, multiposition, Class II live pathogen capabilities. Electron microscopes include SEM and TEM with tomographic capabilities, plus high pressure freezer and freeze substitution and microwave processor.
High Content Screening Facility
The High Content Screening Facility, run from Edinburgh’s Little France campus, includes an Operetta widefield system with high throughput robotic loading, timelapse and temperature control capabilities.
Advanced Imaging Resource
AIR supports imaging ranging from model organisms through to sub cellular structures utilising cutting edge imaging tools and technologies, and works together with ESRIC to provide access to super-resolution optical imaging platforms. Microscopes include confocal, spinning disk confocal, multiphoton, SIM, STORM, OPT, widefield and high content imaging systems, with dedicated image analysis specialist support available.
Dundee Imaging Facility
The Facility provides world-leading core microscopy resources, housing >20 advanced light microscopy systems, including LSCM, WF-decon, SDCM, TIRF,MPLSM, FLIM and 3DSIM technologies. New acquisitions include a diSPIM and AiryScan systems. The Facility also houses electron microscopy and atomic probe microscopy, which are used for research applications in the life and physical sciences.
Centre of Biophotonics
Some of CoB’s light microscopes are commercial
instruments but a significant number of them are innovative techniques developed by groups within the Centre, and therefore, are custom-built setups. Nano- and micro-fabrication techniques within a cleanroom environment and ultrafast techniques including
femtosecond spectroscopy, fluorescence up-conversion
and transient-absorption spectroscopy are available
along with image processing workstations.
Confocal and Advanced Light Microscopy (CALM) Facility
CALM, based in the QMRI at Edinburgh’s Little France campus, offers access to confocal, spinning disk confocal, light-sheet and widefield microscopes with HO-licensed >5dpf zebrafish imaging, laser manipulation, timelapse and multiposition capabilities.
DRP-HCB Light Microscopy Core
The DRP-HCB Light Microscopy Core provides modern and well-maintained equipment for users, particularly in cell biology. Microscopes include inverted and upright confocal, spinning disk confocal and widefield systems with high-speed imaging, multi-position, live cell imaging and cleared sample imaging capabilities.